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Adipogenesis of Adipose Stromal Cells is Reduced by Endothelial Cell Co‐cultivation: Role for Wnt‐signaling
Author(s) -
Rajashekhar Gangaraju,
Roell William,
Traktuev Dmitry,
MerfeldClauss Stephanie,
MacDougald Ormond,
March Keith,
Clauss Matthias
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.49.11
Subject(s) - adipogenesis , wnt signaling pathway , adipose tissue , stromal cell , microbiology and biotechnology , angiogenesis , biology , stromal vascular fraction , chemistry , mesenchymal stem cell , signal transduction , cancer research , endocrinology
Objective: Adipose stromal cells (ASC) display functional and phenotypic overlap with the pericytic cells of microvessels in adipose tissues. Here we addressed the hypothesis that physical proximity of ASC and endothelial cells (EC) regulates the adipogenic potential of ASC. Methods: We investigated the effects of EC on ASC adipogenesis in a 2D‐coculture model and analyzed gene expression changes by real time RT‐PCR. Adipogenesis was determined by quantification of lipid vesicles or increased gene expression of alpha lipase and PPAR‐gamma. Results: A significant decrease in adipogenic differentiation was observed in ASC when cocultured with EC but not in cocultures of ASC with fibroblasts. The majority of molecules involved in Wnt signal transduction were found to be upregulated in coculture conditions with Wnt1 and Wnt4 being most prominent. Using short interfering duplex RNA (siRNA) against Wnt1 in ASC‐EC‐cocultures promoted the adipogenic differentiation. Conclusion: These data identify the close proximity between EC and ASC as a negative regulator of adipogenesis and suggest that perivascular pericytes may protected against adipogenesis. Expression and functional studies further indicate a major role of Wnt signaling proteins in this activity and suggest these molecules as novel candidates to understand regulation of adipogenesis and angiogenesis in adipose tissues.