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Expression pattern of microsomal and soluble epoxide hydrolase in mouse brain
Author(s) -
Marowsky Anne,
Arand Michael
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.479.38
Subject(s) - epoxide hydrolase 2 , choroid plexus , tyrosine hydroxylase , biology , epoxide hydrolase , cerebellum , microbiology and biotechnology , microsomal epoxide hydrolase , hippocampus , lesion , neuroscience , central nervous system , enzyme , pathology , dopamine , biochemistry , microsome , medicine
Not much is known about expression and function of soluble (sEH) and microsomal (mEH) epoxide hydrolase in the brain. In rat brain sEH expression is only detectable in small quantities under normal conditions with a marked increase in spontaneously hypertensive rats. mEH expression is found around Alzheimer plaques and in epileptic and tumor brain tissue in humans and rats, suggesting a lesion‐induced upregulation. We used immunohistochemistry and Western blots to investigate the distribution pattern of the two enzymes in adult mouse brain. Interestingly, the cellular distribution is almost complementary with the sEH exclusively expressed in glia cells of the fore brain while the mEH is found in specific subtypes of neurons, endothelial cells of cerebral vessels and in cells of the choroid plexus. The mEH is particularly abundant in CA3 pyramidal cells of the hippocampus, striatal and central amygdalar neurons and granule cells of the cerebellum. In addition, motor neurons in the brain stem express mEH. While mEH expression in the choroid plexus is in line with the role of the enzyme in detoxification, the neuronal expression pattern implicates an additional function perhaps in signal transmission. sEH expression in astrocytes is consistent with the postulated function of the enzyme in local blood flow regulation.