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BLOOD PRESSURE AND RENAL FUNCTIONS IN MALE AND FEMALE sEH KNOCKOUT MICE
Author(s) -
Yan Qingshang,
Raksaseri Promporn,
Weldon Steven M.,
Kabcenell Alisa K,
Hebert Steven,
Wang Tong
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.479.34
Subject(s) - chemistry , medicine , endocrinology , blood pressure , renal function , urine
We investigated blood pressure (MBP), plasma electrolytes, acid‐base and renal functions in sEH +/+ and sEH −/− mice. In anesthetized male, MBP, GFR, and Na + and K + excretions were significantly reduced in sEH −/− than in sEH +/+ . MBP was 87.9 vs. 107.0 mmHg, GFR was 0.57 vs. 0.81 ml/min/100g BW, EK was1.06 vs. 0.41 uEq/min/100g BW and FEK was 32.76 vs. 16.72%, respectively. The 24‐hour urine and ENa were also reduced in male sEH −/− compared to sEH +/+ , indicating reduced MBP in male sEH −/− is not due to reduction of salt and water absorption in kidneys. In female mice, there were no significant differences in MBP, GFR, and ENa and EK. MBP was 96.6 vs. 101.4 mmHg, GFR was 0.98 vs. 0.88 ml/min/100g BW, EK was 0.69 vs. 0.74 uEq/min/100g BW and FEK was 15.5 vs. 22.3%, respectively. The 24‐hour urine and ENa were not significantly different between female sEH −/− and sEH +/+ . All plasma electrolytes and acid‐base parameters were in the normal range, with no difference between all groups of mice. We conclude that male sEH −/− exhibit lower MBP and GFR and reduced ENa and EK with normal plasma electrolytes and acid‐base balance. Female sEH −/− mice show no significant changes in MBP, renal functions and acid‐base parameters, suggesting that EET activity may be more important in males in lowering blood pressure than in females and the reduction of blood pressure in anesthetized males is more likely via vascular effects of increased EETs in sEH −/− mice.