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Cardiac effects of pulmonary influenza A viral infection
Author(s) -
Bradbury J Alyce,
Carey Michelle A,
Seubert John M,
Myers Page,
Rouse Clay,
Edin Matt,
DeGraff Laura M,
Germolec Dori,
Zeldin Darryl C
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.479.1
Subject(s) - medicine , nasal administration , cardiac function curve , saline , influenza a virus , tumor necrosis factor alpha , phosphorylation , p38 mitogen activated protein kinases , immunology , endocrinology , virus , cardiology , mapk/erk pathway , heart failure , biology , biochemistry
Rationale: Clinical studies have shown a causal relationship between influenza A viral infection (IAV) and cardiovascular disease. Methods: C57BL6 mice were infected intranasally with 200 pfu of Hong Kong influenza A (H3N2) virus while control mice received intranasal saline. Body weight and temperature were recorded daily as indicators of morbidity. On day 4, echocardiograms were performed to assess cardiac dimensions and fractional shortening. Hearts were isolated and perfused in a Langendorff mode to measure contractile function (LVDP). Cytokines and protein kinases were analyzed using Bioplex arrays. Results: IAV induced significant weight loss and changes in body temperature. Echocardiograms showed a reduction in % fractional shortening in the IAV group compared to control (p<0.05). In perfused hearts, there was an increase in post‐ischemic LVDP in IAV vs. controls (p<0.05). Serum IL‐6 levels were elevated on days 2–4, serum TNF‐αlevels were elevated on day 2, phospho‐Akt (pSer 473 ) was suppressed on day 1, and phosphorylation of p38 MAPK (pThr 180 /pTyr 182 ) was increased on day 4 in IAV group relative to controls (all p<0.05). Conclusion: We postulate that improved functional recovery may be due to a preconditioning‐like effect on the heart induced by IL‐6 and TNF‐α. This research was supported by the Intramural Research Program of the NIH, NIEHS.