Premium
Lack of Sex Differences in Muscle Stem Cell Transplantation for Cardiac Repair
Author(s) -
Drowley Lauren,
Okada Masaho,
Payne Thomas,
Botta Gregory,
Oshima Hideki,
Tobita Kimimasa,
Keller Bradley,
Huard Johnny
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.466.2
Subject(s) - stem cell , transplantation , myocardial infarction , medicine , myocyte , cardiac function curve , skeletal muscle , population , cardiac muscle , cardiomyopathy , bone marrow , pathology , andrology , biology , heart failure , microbiology and biotechnology , environmental health
Cellular transplantation of stem cells is a potential method of repairing damaged myocardium. We identified a novel population of muscle‐derived stem cells (MDSCs) that can repair cardiac muscle. Sex differences have been seen in cardiovascular diseases and cellular therapy. Differences in regenerative capacity of male and female MDSCs have been observed in skeletal muscle and bone. In this study we compared male and female MDSCs to determine if there was a sex difference for cardiac repair. MDSCs were implanted into the hearts of both a cardiomyopathy mouse model and a myocardial infarction model. In the cardiomyopathy model, five populations of male and female cells were injected into both male and female mice. No differences were found with either host sex or cell sex in engraftment or blood vessels. For the injury model, cells were divided into 4 groups – high and low engrafting cells from both sexes, and injected into SCID mice after infarction to assess function at six weeks. No difference was seen in function between the groups, though all cell‐injected groups were significantly better than PBS controls. The infarcted hearts were stained for scar tissue, blood vessels, and engraftment, and no difference was seen between the cell groups. Though our lab has previously shown sex differences with MDSCs in skeletal muscle and bone, this does not appear to be the case in cardiac muscle. Since MDSCs seem to repair the heart through secretion of VEGF and chemoattracting host cells rather than directly regenerating the myocardium, this could explain why no difference was observed between male and female cells. This work was funded by grants to J. Huard from the MDA, the NIH (1U54AR050733‐01, HL 069368) and L. Drowley from the NIH (T32 EB001026‐05).