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Matrix Metalloproteinase (MMP)‐1 Induces Migration and Proliferation of Lung Epithelial Cells
Author(s) -
Herrera Iliana,
Cisneros Jose,
Ramírez Remedios,
Garcia Jorge,
Selman Moises,
Pardo Annie
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.464.8
Subject(s) - mmp1 , transfection , wound healing , matrix metalloproteinase , cell growth , cell migration , chemistry , microbiology and biotechnology , cell , biology , cell culture , immunology , gene expression , biochemistry , genetics , gene
MMP1 is a multifunctional protease that cleaves fibrillar collagens and process a number of non‐matrix components. Studies in our laboratory have demonstrated that MMP1 is upregulated in idiopathic pulmonary fibrosis. However, the protein is expressed in alveolar epithelial cells and it is virtually absent in the lung interstitium and fibroblastic foci where collagens are being accumulated. In this study we explored the putative function of epithelial expression of this enzyme. Mouse lung epithelial cells (MLE‐12) were transfected with human MMP1 linked to the expression vector pQCXIP. Control cells were transfected with the vector. Cell migration was analyzed using Boyden chambers covered with collagen and by in vitro wound healing assay using epidermal growth factor as chemoattractant. Cell proliferation was evaluated with WST‐1 reagent. MMP1 transfected epithelial cells showed a significant increase in cell migration (DO: 0.39±0.4 vs 0.24±0.2; p<0.05). In the wound healing assay MMP1 transfected cells covered 100% of the wound at 36 h, compared with 60% of the control epithelial cells. Likewise, MMP1 transfected cells exhibited a significant increase of proliferation at 36 h (300±20% vs 254±11%; p<0.05) and at 48 h (394±33% vs 300±11%; p<0.01). These findings suggest that MMP1 may have a role in lung epithelial cell migration and proliferation. Funded by: UNAM, Grant SD1.PTID.05.6