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Oxidative Stress in Premature Infants
Author(s) -
Friel James Kenneth,
Chiu Aaron,
DiehlJones William,
Roberts Jackson
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.453.4
Subject(s) - medicine , creatinine , oxidative stress , gestational age , urine , gestation , birth weight , physiology , gastroenterology , pregnancy , genetics , biology
We hypothesized that early infant mortality in the premature infant (< 37 weeks gestation), which is normally high, as well as increased morbidity reflects the difficulty of adaptation to oxygen. Therefore forty PT infants (birth weight: 1146 ± 38 g; gestational age 28 ± 0.3 wks) were followed biweekly once oral feeds were introduced. Oxidative stress was quantified by measuring dinor, dihydro metabolites of F2‐isoprostanes and isofurans in urine by liquid chromatography/tandem mass spectrometry. The precision of the assay is ± 5% and the accuracy is 80%. For F2‐isoprostanes results were : 0–2 wks, 147 ± 12; 3–4 wks, 213 ± 34; 5–6 wks, 203 ± 16; 7–8 wks, 246 ± 38; 9–10 wks, 240 ± 38; > 10 wks, 191 ± 47 (ng/mg creatinine, mean ± SE). The normal levels from 40 adults are 38.1 ± 19.1 ng/mg creatinine. For isofurans: 0–2 wks, 17 ± 2; 3–4 wks, 20 ± 3; 5–6 wks, 24 ± 3; 7–8 wks, 24 ± 5; 9–10 wks, 25 ± 5; > 10 wks, 31 ± 8 ng/mg creatinine. The normal levels in adults (n=22) are 4.35 ± 1.9 ng/mg creatinine. Ability to resist oxidative stress was assessed with ORAC: baseline: 6.6 ± 0.2 and at discharge, 6.7 ± 0.1 (healthy premature 6 mos, 6.6 ± 1.1; full term 4 & 12 M, 9.9 ± 0.6; adult 9.3 ± 0.9. These data suggest that the PT human infant is under severe oxidative stress and it may be appropriate to consider routine antioxidant supplementation for the first months of life.