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Expression of lipid metabolism genes in skeletal muscle after weight loss in post‐obese insulin resistant dogs
Author(s) -
Leray Veronique,
Serisier Samuel,
Nguyen Patrick
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.444.8
Subject(s) - endocrinology , medicine , perilipin , skeletal muscle , insulin resistance , lipogenesis , lipolysis , lipid metabolism , insulin , weight loss , biology , carbohydrate metabolism , adipose tissue , obesity
Overweight and obesity are associated with insulin resistance, and impaired lipid and glucose metabolism in insulin target tissues. We have previously showed in dogs that a weight loss was accompanied by changes in the expression of lipogenesis and lipolysis in fat and liver. The aim of this study was to evaluate the effect of weight loss on insulin sensitivity and expression of these genes in skeletal muscle. Obese dogs were given a hypoenergetic diet in order to induce weight loss. When dogs were obese and then when they had reached their healthy body weight, insulin sensitivity was assessed using the euglycemic hyperinsulinemic clamp. mRNA expression of IRS‐2, SREBP, ACC, FABP, ADRP, PEPCK, perilipin and HSL was semi‐quantified using real time RT‐PCR in skeletal muscle. Dogs' body weight was 25 % lower at the end of the slimming period compared to obese state. Insulin sensitivity was higher (+ 40 %). In skeletal muscle, the mRNA expression of perilipin and ADRP was significantly lower (− 50 %). The expression of genes involved in lipid metabolism did not change. These results showed a decrease in perilipin and ADRP expression, which could reflect the lower lipid content in skeletal muscle after weight loss. They also show that weight loss did not affect the expression of lipid metabolism genes in skeletal muscle, although insulin sensitivity recovery, contrary to our previous results in fat and liver.