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Promoter architecture dynamically modulates transcription factor binding and transcriptional output over time
Author(s) -
Lieb Jason Dillon,
Hanlon Sean,
Lickwar Colin
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.400.2
Subject(s) - transcription factor , rap1 , biology , promoter , transcriptional regulation , taf2 , genetics , dna binding site , binding site , sp3 transcription factor , microbiology and biotechnology , gene , gene expression
Our laboratory studies relationships between chromatin, transcription factor binding, and transcriptional output in S. cerevisiae, C. elegans and human cells. Here, I will present a study in which we experimentally determined the genomic distribution of the yeast transcription factor Rap1 during vegetative growth, respiratory growth and throughout meiosis and sporulation in yeast. Simultaneously, we monitored the expression of the genes downstream of binding events. In short, all simple combinations of binding dynamics and transcriptional output were observed. Even though within the same cell individual targets downstream of Rap1 binding may be strongly repressed or strongly activated, we demonstrate that Rap1 is required for both types of transcriptional outcome by depleting the transcription factor from cells. While there is no simple relationship between Rap1 binding and transcriptional activity, differences in transcriptional outcome can be partially explained by the presence of additional transcription factor binding motifs and the type of Rap1 motif contained within the promoter of target genes. We are also developing a system in yeast to probe transcription factor binding dynamics genome‐wide in yeast, and will report progress in that area as well.