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Defining Cardiac Cell Populations and Their Interactions
Author(s) -
Baudino Troy A,
Banerjee Indroneal,
Intwala Arti
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.386.4
Subject(s) - ventricle , myocyte , biology , cell sorting , muscle hypertrophy , cell , population , cell type , cardiac muscle , microbiology and biotechnology , medicine , anatomy , endocrinology , genetics , environmental health
Cardiac fibroblasts, myocytes, endothelial cells and vascular smooth muscle cells are the major cellular constituents of the heart. Our recent studies have allowed us to observe alterations in the myocardial cell populations during development, in the adult animal and during disease using both surgical and genetic models. Whole hearts were isolated from mice during the neonatal and adult stages and single cell suspensions were prepared. Heterogeneous cell populations were immunolabeled for specific cell types and examined using Fluorescence Activated Cell Sorting (FACS) analyses. In addition, the left ventricle, right ventricle and septa were isolated, fixed and sectioned for morphometric analyses. These same cardiac regions were analyzed using FACS analyses. We observed that the adult murine myocardium is composed of approximately 56% myocytes, 27% fibroblasts, 7% endothelial cells and 10% vascular smooth muscle cells. Moreover, our morphometric and FACS data demonstrated similar percentages in both analyses. Furthermore, our current studies have demonstrated dramatic cardiac cell population shifts in IL‐6‐ and periostin‐deficient mice, as well as in a pressure overload hypertrophy model. Taken together, these data have enabled us to establish a homeostatic model for the myocardium that can be compared to other genetic and cardiac disease models.