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Computational imaging of heart morphogenesis in vivo
Author(s) -
Rongish Brenda J,
Cui Cheng,
Lansford Rusty D,
Little Charles D
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.386.1
Subject(s) - endoderm , primitive streak , biology , embryo , progenitor cell , heart development , fate mapping , morphogenesis , anatomy , microbiology and biotechnology , in vivo , stage (stratigraphy) , compartment (ship) , embryogenesis , stem cell , embryonic stem cell , gastrulation , genetics , gene , paleontology , oceanography , geology
Cardiac progenitor cells were followed in time and space from HH Stage 4 through Stage 12 in avian embryos to determine their position fate. Embryos were electroporated at HH Stages 3‐3+ with H2B‐GFP, a fluorescent nuclear marker, and subjected to wide‐field time‐lapse imaging. Thus, cardiac precursor cells were tracked from the primitive streak into the tubular heart, where their dorsal‐ventral and anterior‐posterior fates were determined. The data demonstrate the existence of a continuous heart field, expanding as a wide arc of progenitors at HH stage 4. Sub‐groups or anatomical bands of heart precursor cells change their relative positions as endoderm folding proceeds. Moreover, expression of the putative cardiac molecular markers, Nkx‐2.5 and Bmp‐2 does not fully overlap with a subset of cardiac precursors until HH Stage 10, the tubular heart stage. These data resolve the origin of cardiac progenitor cells and redefine the primary heart forming region in a warm‐blooded embryo. Supported by an AHA fellowship (CC) and the G. Harold and Leila Y. Mathers Charitable Foundation (CDL, BJR).