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Arachidonic acid increases MCF‐10A and MCF‐10CA1 motility dependent on PPAR‐gamma
Author(s) -
Carter Jennifer C.,
Church Frank C.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.321.5
Subject(s) - mcf 7 , arachidonic acid , motility , chemotaxis , cell culture , medicine , endocrinology , peroxisome proliferator activated receptor , receptor , biology , cancer research , chemistry , microbiology and biotechnology , cancer cell , biochemistry , cancer , genetics , human breast , enzyme
Recently, a high‐fat Western diet has been linked to an increase breast cancer risk. This high‐fat diet has an increased of omega‐6 (ω–6) poly‐unsaturated fatty acids (PUFAs) compared to omega‐3 (ω–3) PUFAs. We are investigating the role of one such ω‐6 PUFA, arachidonic acid (ArA), in breast cancer cell motility. ArA has been shown to be involved in increased cell motility and survival. Interestingly, ArA has also been shown to activate peroxisome‐proliferator activated receptor‐gamma (PPAR‐γ), a protein involved in adipocyte differentiation and plasminogen activator (PA) system regulation. We used two cell lines, MCF‐10CA1, an invasive carcinoma line and normal breast epithelial line, MCF‐10As, cells to study the effects of ArA. Chemotaxis to epidermal growth factor (EGF) was greatly increased in MCF‐10CA1 cells compared to the normal MCF‐10As. Arachidonic acid treatment increased chemotaxis in both cell lines about 2‐fold. Treatment with ArA increased uPA activity MCF‐10A cells, with a less dramatic increase in MCF‐10CA1 cells. To determine if ArA was acting on PPAR‐γ, we pretreated the cells with GW9662, a PPAR‐γ antagonist, and saw the ArA mediated increase in motility was abolished in both cell lines. This data suggests ArA increases cellular motility of normal and malignant cells through alterations in PA system components, through a PPAR‐γ dependent mechanism.