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Effect of folate deprivation on WNT signaling pathway in human colon cell lines
Author(s) -
Huang Min,
Chen Hong
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.311.4
Subject(s) - wnt signaling pathway , dkk1 , axin2 , dna methylation , bisulfite sequencing , methylation , cell culture , epigenetics , microbiology and biotechnology , cpg site , colorectal cancer , chemistry , signal transduction , biology , gene , gene expression , cancer research , cancer , biochemistry , genetics
Human epidemiology studies and designed animal experiments have shown both timing and dose are critical for folate relation with colorectal cancer. The molecular mechanism of folate effect is not fully understood. In our study, we examined gene expression of several specific genes of wnt signaling pathway in human colonic cells lines at different stages with or without folate in culture media. Cell proliferation was inhibited at around day 8 after folate depletion. Quantitative real‐time RT‐PCR results showed that mRNA level of DKK1 was increased whereas AXIN2 and NKD1 decreased with time in SW480. No significant changes were observed in DLD1. Methylation sensitive PCR and bisulfite sequencing were used to examine whether DNA methylation level of these genes altered. The putative CpG island of DKK1 analyzed in this study is totally unmethylated in SW480 and hypermethylated in DLD1 and no significant changes were observed after folate depletion. In conclusion, folate deprivation altered expression of DKK1 in wnt pathway in SW480 human colonic cancer cell line and the effect is not due to DNA methylation levels.