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Effect of energy balance on gene expression in bovine mammary tissue
Author(s) -
Moyes Kasey M,
Drackley J K,
Morin D E,
RodriguezZas S L,
Everts R E,
Lewin H A
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.294.4
Subject(s) - unigene , biology , gene expression , gene , lactation , complementary dna , mammary gland , gene expression profiling , rna extraction , reference genes , microbiology and biotechnology , andrology , genetics , transcriptome , breast cancer , cancer , pregnancy , medicine
Objectives were to compare mammary tissue gene expression profiles between lactating cows subjected to dietary‐induced negative energy balance (NEB; n = 5/treatment) and cows fed ad libitum (AL) to maintain positive energy balance. NEB cows were feed restricted (FR) to 60% of calculated net energy for lactation requirements for 5 d. After 5 d of FR, both rear mammary quarters of all cows were biopsied for RNA extraction. A 13,257 oligonucleotide (70‐mers) array and qPCR were used for transcript profiling. Annotation was based on similarity searches using BLASTN against human, mouse and bovine RefSeq, human, mouse, and bovine UniGene, and bovine TIGR. Cy3‐ and Cy5‐labelled cDNA from mammary tissue and a reference standard were used for hybridizations. NEB resulted in 299 differentially expressed genes (P < 0.01). Among genes downregulated with NEB (156 genes total), Ingenuity Pathway Analysis® identified cell cycle (5), cell morphology (12), and cellular development (18) as some of the most enriched molecular functions. Genes upregulated by NEB (141 genes total) were associated with amino acid metabolism (11), small molecule biochemistry (18), gene expression (10) and lipid metabolism (8). Results indicate that NEB alters mammary gene expression. Project was funded by Section 1433 Animal Health and Disease, University of Illinois #ILLU‐538‐981, and NRI‐USDA #2007‐35204‐17758.