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Mouse models to study the role of CD34 in allergy and inflammatory diseases
Author(s) -
Blanchet MarieRenee,
Maltby Steven,
Bennett Jami,
McNagny Kelly
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.238.3
Subject(s) - cd34 , immunology , inflammation , haematopoiesis , biology , mast cell , stem cell , cancer research , microbiology and biotechnology
The CD34 antigen is widely used as a stem cell marker. However, the exact role of CD34 expression is still misunderstood. Our lab is currently using mouse models of allergy and inflammation induced in CD34‐null or chimeric mice in order to elucidate the role of CD34 expression in inflammation. Asthma, hypersentitivity pneumonitis (HP), experimental animal encephalomyetis (EAE) or arthritis was induced in CD34‐null and wild type mice. These pathologies are characterized by activation of various CD34‐expressing cells. Disease progression and cell recruitment and function were compared between wild type and CD34‐deficient mice. In lung inflammation, CD34‐null mice are protected against development of disease and show various cell trafficking defects. In EAE, mast cells accumulate in the CNS of CD34‐null mice, possibly through an emigration defect. Finally, in arthritis, CD34‐null mice show higher susceptibility to disease due to lack of CD34 on vascular endothelia. These results suggest a role for CD34 in hematopoietic cell trafficking, either through CD34 expression on inflammatory cells or vascular endothelia. Also, CD34 expression on vascular endothelia is involved in progression of arthritis. The use of these mouse models in combination with CD34‐null mice has allowed us to better understand the role of CD34 expression in development of inflammation and allergy.