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Sall genes regulates limb patterning through modulation of region‐specific Hox activities in mice
Author(s) -
Kawakami Yasuhiko,
Nishinakamura Ryuichi,
Belmonte Juan Carlos Izpisua
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.230.6
Subject(s) - hox gene , biology , gene , transcription factor , zinc finger , genetics , phenotype , microbiology and biotechnology , organogenesis , zinc finger transcription factor
Sall genes encode zinc finger transcription factors, and Sall1 and Sall4 are causative genes for human syndromes, such as Towns‐Brocks syndrome and Okihiro syndromes, respectively. Patients with these syndromes show multiple defects including those in limbs. In order to understand the molecular mechanisms by which Sall genes control limb development, we have analyzed mice with targeted mutations in Sall1 and Sall3. While a single knockout of Sall1 and Sall3 shows subtle defects, double mutants exhibited severe defects in autopod development. We found that Sall1 interacts with Hoxa13 and Hoxd13, important genes controlling proper development of the autopod. Further analyses suggest that the coordinated action of Sall and Hox regulates target gene expression and patterning of the autopod. Our study provides molecular insights into how region‐specific morphogenesis is regulated in the autopod. Supported by NIH (5 R01 HD034538‐10) and Grant‐in‐aid by the Ministry of Education, Culture, Sports, Science, and Technology, Japan.