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Colored Potato Bioactive Compounds Suppress Proliferation and Induce Apoptosis in Both Androgen‐Dependent and –Independent Prostate Cancer Cell Lines
Author(s) -
Reddivari Lavanya,
Vanamala Jairam,
Safe Stephen H.,
Miller J. Creighton
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.158.3
Subject(s) - lncap , gallic acid , apoptosis , chemistry , kinase , cell growth , cancer research , biochemistry , biology , cancer cell , antioxidant , cancer , genetics
We recently reported that colored potato extracts induce apoptosis in LNCaP (androgen‐dependent) and PC‐3 (androgen‐independent) prostate cancer cell lines via caspase‐dependent and ‐independent pathways. In order to identify the most active growth inhibiting and pro‐apoptotic compounds in the potato extract, we have further investigated the effects of different phenolic compounds (chlorogenic acid, caffeic acid, gallic acid, catechin and malvidin) and glycoalkaloids (α‐ chaconine and solanine) found in these extracts on PC‐3 and LNCaP cells. α‐ chaconine (5 μg/ml) and gallic acid (10 μg/ml) showed potent antiproliferative properties and increased levels of the cyclin‐dependent kinase inhibitor p27 in both cell lines. Gallic acid decreased cyclin D1 levels in LNCaP cells. The growth inhibitory effects of α‐ chaconine were potentiated by SP600125, a c‐jun N‐terminal kinase (JNK) inhibitor, and atractyloside, an ADP phosphorylation inhibitor in both cell lines. Both α‐ chaconine and gallic acid induced caspase‐dependent apoptosis through PARP cleavage and activation of caspase‐3 in LNCaP cells. However, induction of apoptosis in PC‐3 cells was caspase‐independent. These results suggest that apoptosis induced by whole potato extracts in prostate cancer cell lines may be in part due to α‐ chaconine and gallic acid. CSREES special research grant.