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Rhodiola extract inhibits adipocyte differentiation in 3T3‐L1 cells
Author(s) -
Lee OKHwan,
Kwon YoungIn,
Apostolidis Emmanouil,
Shetty Kalidas,
Lee BooYong,
Kim YoungCheul
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.148.6
Subject(s) - pentose phosphate pathway , rhodiola , adipogenesis , adipocyte , glucose 6 phosphate dehydrogenase , dehydrogenase , 3t3 l1 , downregulation and upregulation , western blot , biochemistry , chemistry , adipose tissue , biology , enzyme , microbiology and biotechnology , salidroside , glycolysis , gene , pharmacology
Adipocyte differentiation is a key process in the development of obesity as well as in normal development and function of adipose tissue. We have previously shown that Rhodiola extract high in phenolics dose‐dependently inhibited the enzyme activity of both glucose‐6‐phosphate dehydrogenase (G6PDH), the rate‐limiting enzyme of the pentose phosphate pathway (PPP), and proline dehydrogenase (PDH) in differentiating 3T3‐L1 preadipocytes. The present study is designed to investigate whether the inhibitory effects of Rhodiola extract on enzyme activities correlate with gene expression pattern and further PPARγ mRNA and protein expression, a key adipogenic transcription factor, by semi‐quantitative RT‐PCR and Western blot analysis. 3T3‐L1 preadipocytes were treated with various concentrations of Rhodiola extract for up to 8 days following standard induction of differentiation. Rhodiola treatment significantly inhibited expression of G6PDH and PDH mRNAs as well as mRNA and protein expression in a dose‐dependent manner. These results indicate that Rhodiola extract can inhibit adipogenesis through a mechanism(s) that involves direct downregulation of PPARγ gene expression or via modulation of pentose phosphate pathway linked to PDH, which may be important for cellular energy and redox environment in adipocytes.