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Impact of selenium and selenoproteins on regulation of glucose homeostasis and insulin sensitivity in mice
Author(s) -
Labunskyy Vyacheslav M.,
Hatfield Dolph L.,
Gladyshev Vadim N.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.146.5
Subject(s) - selenium , gpx1 , medicine , endocrinology , selenoprotein , glucose homeostasis , homeostasis , diabetes mellitus , insulin , genetically modified mouse , chemistry , transgene , biology , insulin resistance , oxidative stress , glutathione peroxidase , biochemistry , catalase , gene , organic chemistry
Selenium is an essential trace element, which has been shown to be effective in cancer prevention. Whereas increased levels of selenium in the diet have important health benefits, recent clinical trials have demonstrated that supplemental intake of selenium above the adequate level raises the risk of type 2 diabetes mellitus. However, the molecular mechanisms for the effect of dietary selenium on the development of this disease are not well understood. In the present study, we examined the contribution of selenoproteins to increased risk of developing diabetes. C57BL/6J mice (n=6–8 per group) were fed either selenium deficient Torula yeast based diet or diets supplemented with 0.1 and 0.4 ppm selenium. Our data show decreased insulin sensitivity in mice maintained on selenium supplemented compared to selenium deficient diets. As expected, we observed elevated levels of stress‐related selenoproteins, such as GPx1 and MsrB1, while expression of housekeeping selenoproteins, such as TR3, was not significantly changed upon increased concentration of selenium in the diets. In addition, our preliminary data show a dysregulation of glucose homeostasis in transgenic mice encoding an i 6 A − mutant Sec tRNA, which are characterized by reduced synthesis of selenoproteins. Together with previous reports showing impaired insulin sensitivity in mice that overexpress GPx1, these data suggest a possible role for the stress‐related selenoproteins in regulation of glucose homeostasis and insulin sensitivity in mice.