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Selenium status in healthy adults in a longitudinal study using both traditional biochemical and molecular biology‐based biomarkers
Author(s) -
Sunde Roger A,
Paterson Elaine,
Barnes Kimberly M,
Evenson Jacqueline K,
Lovegrove Julie A,
Gordon Michael A
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.146.3
Subject(s) - gpx1 , selenoprotein p , selenoprotein , gpx3 , medicine , selenium , endocrinology , biology , population , glutathione peroxidase , chemistry , oxidative stress , superoxide dismutase , environmental health , organic chemistry
Human Se requirements are currently based on biochemical markers of Se status. Gpx1 mRNA levels can also be used to determine rat Se requirements. We found that Gpx1 mRNA levels decrease in rat blood and that Selh and Sepw1 mRNAs are also highly down‐regulated (<40%). Thus we collaborated with a Reading UK study, which examined effects of vegetable‐fruit‐based juices (with negligible Se <1 μg/d) in 39 subjects (24 female, 15 male, age 45 ± 11, BMI 24.5 ± 3.2). Diet records (5 d) and blood were obtained at 2, 8, 17 and 23 wk. There was no effect of juice on any Se marker, and there was no significant longitudinal effect on Se markers, so time‐point values were averaged. Se intake was 46 ± 9 μg/d for females and 45 ± 11 μg/d for males. Plasma Se values were 1.13 ± 0.04 μmol/L. Plasma Se v Se intake (Se/kcal) were significantly correlated, but neither Gpx3 activity v Se intake nor Gpx3 activity v plasma Se were correlated. Collectively, this indicates that these subjects were on the response curve plateaus. Similarly, blood mRNA levels for Gpx1, Selh, and Sepw1 (highly regulated by Se in rodents) as well as Sepp1, Gpx3 and Gpx4 were also not significantly correlated with plasma Se. Thus selenoprotein mRNAs are readily quantitated in human blood. These molecular markers, however, as well as biochemical and chemical markers, do not suggest that this population is marginal in Se status. (Supported in part by NIH DK74184)