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Effect of cadmium on cellular protein and glutathione synthesis and expression on stress proteins in eastern oysters, Crassostrea virginica Gmelin
Author(s) -
Ivanina Anna,
Cherkasov Anton,
Sokolova Inna
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1239.6
Subject(s) - glutathione , heat shock protein , hsp70 , metallothionein , crassostrea , protein biosynthesis , cadmium , hepatopancreas , oyster , biology , cellular stress response , toxicant , cadmium chloride , biochemistry , microbiology and biotechnology , chemistry , toxicity , ecology , fight or flight response , enzyme , gene , organic chemistry
Cadmium (Cd) is an important toxicant in estuarine and coastal environments which can strongly affect energy balance of aquatic organisms by increasing the organism's basal energy demand and reducing its aerobic capacity. Mechanisms of Cd‐induced increase in basal metabolic costs are not well understood and may involve elevated detoxification costs due to the synthesis of cellular protective proteins and glutathione (GSH). We studied the effects of Cd exposure on protein and GSH synthesis and expression of stress protein (heat shock proteins HSP60, HSP70 and HSP90) and metallothioneins in isolated gill and hepatopancreas (HP) cells of the eastern oysters Crassostrea virginica. Our study showed that exposure to Cd (10–2000 ìM for 4 h) resulted in dose‐dependent increase in the rate of the protein synthesis in oyster cells. GSH synthesis was inhibited by the highest Cd concentration (2 mM) especially in HP cells. Elevated protein synthesis was associated with the increased expression of metallothioneins and heat shock proteins. Stress protein response considerably differed between gill and HP cells. In HP, there was an increase in expression of MT mRNA, but no change in HSP protein level in response to Cd exposure. In gill cells no significant increase in MT expression was found and level of HSP60 and 70 significantly increased in Cd‐exposed cells.