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The Biologic Properties and Ultra‐Microstructure in the Senescence of Endothelial Cell Induced by AngiotensinII
Author(s) -
Bai Xiaojuan,
Shan Haiyan,
Chen Xiangmei
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1238.6
Subject(s) - senescence , apoptosis , flow cytometry , annexin , acridine orange , cell , microbiology and biotechnology , staining , cell growth , cell cycle , umbilical vein , viability assay , human umbilical vein endothelial cell , chemistry , mtt assay , biology , in vitro , biochemistry , genetics
Objective The aim of this study was to investigate the biologic effects and morphology senescence of human umbilical vein endothelial cell (HUVEC) induced by angiotensinII and its possible action mechanism. Methods HUVEC were cultured in vitro and intervened by AngII. The cell living rate was measured by MTT assay; SA‐β‐gal stain and cell cycle analysis were used to identify cell aging status; the early stage apoptosis was detected by flow cytometry with Annexin V‐FITC/PI double staining, morphologic changes and percentage of apoptosis were assayed with acridine orange fluorescence staining and were observed under fluorescent microscope, transmission electron microscopy was used to evaluate the ultra‐microstructure of HUVEC. Results Induction of AngII promoted a growth arrest with phenotypic characteristics of cell senescence, such as enlarged cell shapes, increased senescence‐associated β‐galactosidase (SA‐β‐gal) staining and depressed cell proliferation. Apoptotic number and percentage was increased in senescent cell, which a small subset of the senescent cells showed aberrant morphology such as remarkable nuclear fragmentation or multiple micronuclei. Conclusions Our results suggest cell apoptosis is possibly an important factor for the process of pathologic and physiologic endothelial cells senescence and vascular aging.