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Reguation of oxidative stress by resveratrol in repetitively loaded muscles.
Author(s) -
Ryan Michael James,
Always Stephen E.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1235.7
Subject(s) - resveratrol , oxidative stress , catalase , xanthine oxidase , isometric exercise , chemistry , endocrinology , medicine , biochemistry , enzyme
Resveratrol has been suggested to have several beneficial health effects in lower animals and mammals including anti‐cancer, anti‐viral, anti‐inflammatory, and anti‐aging. The purpose of this study was to examine the effects of dietary supplementaiont with resveratrol on oxidative stress induced in mice by repetitive isometric contractions. We hypothesized that resveratrol supplementation would lower indices of oxidative stress and increase the muscles’ adaptive response to loading. Young adult (3 mo) mice were randomly assigned to either a control (unsupplemented) diet or a diet supplemented with 0.05% resveratrol. The plantar flexors of one limb were activated by 20, five‐second isometric contractions (10v, 100 Hz, 200 ìs pulses) for 3 consecutive days. The contralateral limb served as the intra‐animal control. We measured H 2 O 2 production, the GSH/GSSG ratio, aconitase, xanthine oxidase (XO), GPx, Catalase, Mn SOD and CuZn SOD activity, along with mRNA for GPx, Catalase, Mn SOD and CuZnSOD immediately after the last exercise session in control and loaded muscles. The data suggest a link between resveratrol supplementation and the attenuation of increased XO activity associated with repetitive loading. Furthermore, the decrease in XO activity attenuates the increase in oxidative stress related to repetitive loading in mice.