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Analysis of Cardiac Norepinephrine (NE) Transporter (NET) mRNA after removal of the stellate ganglia.
Author(s) -
Esfahanian Mohammad,
Wehrwein E A,
Yoshimoto M,
Osborn J W,
Novotny M,
Babankova D,
Swain G M,
Kreulen D L
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1230.2
Subject(s) - ganglionectomy , messenger rna , sympathetic denervation , stellate ganglion , denervation , norepinephrine transporter , medicine , endocrinology , norepinephrine , hepatic stellate cell , chemistry , biology , pathology , dopamine , gene , biochemistry , alternative medicine
NET is an important protein in the regulation of catecholamines, particularly NE, and its dysfunction has been linked to certain cardiovascular diseases. To evaluate the contribution of the sympathetic innervation from the stellate ganglia as a source of NET in the heart, we compared by qPCR NET mRNA in the heart in control rats to rats after removal of stellate ganglia. NET mRNA was present in heart homogenate, with atria expressing more NET mRNA than ventricles (p<0.05, n=4–6). Adult, male rats underwent bilateral stellate ganglionectomy (SGX) or sham surgery and hearts were assayed by chamber two weeks later. To confirm successful denervation, NE content was measured by HPLC and was reduced uniformly by 85.96% in all heart chambers (p<0.001, n=4–5). Although NE was depleted, there was a significant increase in NET mRNA (p<0.05, n=5) in the left atria while no differences were seen in any other chambers (p>0.05, n=4–6). The presence of NET mRNA after SGX suggests that NET mRNA is present in non‐stellate sources and is regionally increased by removal of the sympathetic innervation. P01HL79687, AHA predoctoral fellowship (EW).