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Plasma potassium concentrations after severe controlled hemorrhage in inbred rat strains
Author(s) -
Klemcke H. G.,
Kheirabadi B.,
Ryan K. L.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1227.4
Subject(s) - basal (medicine) , potassium , hemorrhagic shock , resuscitation , inbred strain , strain (injury) , medicine , shock (circulatory) , endocrinology , homeostasis , andrology , biology , zoology , chemistry , surgery , biochemistry , gene , organic chemistry , insulin
Arterial plasma potassium concentrations [K + ] a increase after severe hemorrhage (Shires et al., Ann Surg 176:288, 1972)‐‐ which is consistent with decreased activity of ATP‐dependent Na/K ATPase (Darlington and Gann, J Trauma 58: 1, 2005)‐‐ and are significantly higher in non‐survivors vs survivors after hemorrhage (Torres et al., Am J Physiol 286: H1811, 2004). Eight‐fold differences in survival time were detected among 15 inbred rat strains after a controlled hemorrhage without resuscitation (Klemcke et al., Shock , in press, 2007). To assess potential mechanisms of these differences, [K + ] a was measured at the beginning (basal) and end (final) of hemorrhage in those 15 inbred strains. Rats (n = 8–11/strain)were catheterized and, 24 hours later, 55% of the blood volume was removed during a 26 min period from conscious unrestrained animals. Rats were then observed for 6 hr or until death. Using average values for each strain, there were correlations between survival time and basal [K + ] a (r= 0.52; P=0.04), final [K + ] a (r= −0.70; P<0.01) and changes in [K + ] a (final ‐ basal; r= −0.83; P<0.01). In support of these correlations, analysis of all strains via ANOVA revealed that longer‐lived strains had higher basal [K + ] a , lower final [K + ] a , and diminished changes in [K + ] a (P<0.01) than did strains with shorter survival times. These results suggest genetic differences in mechanisms associated with both potassium homeostasis and hemorrhage‐induced alterations.