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Nitrosative Stress in an Ovine Model of Acute Lung Injury
Author(s) -
Esechie Aimalohi,
Enkbaatar Perenlei,
Horvath Eszter,
Jonkam Collette,
Hamahata Atsumori,
Traber Lillian,
Traber Daniel
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1227.14
Subject(s) - smoke inhalation , nitrotyrosine , smoke inhalation injury , burn injury , nitric oxide , anesthesia , medicine , nitrite , smoke , inhalation , lung , nitric oxide synthase , andrology , chemistry , surgery , nitrate , organic chemistry
AIM: elucidate changes involved in nitrosative stress using an ovine model of ALI. METHOD: Sheep (n=5 per group) were surgically instrumented for chronic study. 5 days after surgery, the animals were randomly allocated as follows: Injury (instrumented; 3rd degree 40% total body surface area flame burn and insufflation with 48 breaths of cotton smoke under deep anesthesia) and sacrificed at the following time points post‐injury: T=4hr, 8hr, 12hr, and 24hr; control (instrumented; no injury). Data is reported as mean ± SEM. RESULTS: Pulmonary gas exchange decreased 24h post burn and smoke injury (p < 0.05) and correlated with increased lung water content at 18h and 24 h (p< 0.05). Inducible nitric oxide synthase (iNOS) expression increased after injury compared with the control groups; peak iNOS expression was at 12h post burn and smoke inhalation (p < 0.05 vs control). Plasma nitrate/nitrite (NOx) correlated with protein expression: plasma NOx values increased 12h post injury (p = 0.046). 3‐nitrotyrosine (index of reactive nitrogen species) increased after burn and smoke inhalation compared to control (p < 0.05, n=5). DNA damage was assessed by measuring repair enzyme, poly (ADP) ribose polymerase (PARP) which increased in the injury group (p < 0.05 vs control). CONCLUSION: Therapeutic interventions to mediate excess free radical production could ameliorate impaired pulmonary function associated with ALI.