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Repeated exposures to hyperbaric air suppress neurite outgrowth in PC12 cells
Author(s) -
Isada Tetsuro,
Hirose Munetaka,
Murata Eri,
Shigemi Kenji
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1223.2
Subject(s) - neurite , nerve growth factor , medicine , stimulation , anesthesia , tropomyosin receptor kinase a , central nervous system , neuroscience , biology , in vitro , receptor , biochemistry
Objective: Scuba divers are at risk of central nervous system damage under hyperbaric exposure, which would directly causes lesions of neural cells. Method: After NGF stimulation, PC12 cells were compressed for 30 minutes as single exposure, and repeated exposures for 30 minutes were applied 3 times with intervals 3,15,30 minutes. Then neurite outgrowth was observed. Downstream signals of NGF (TrkA and ERK) were also examined under single and repeated exposures. Result: Repeated exposures to hyperbaric air with either 3 or 15 min interval suppresses NGF‐stimulated neurite outgrowth. Although TrkA showed no change in autophosphorylation, repeated exposures with 3 min interval reduced ERK protein expression significantly. Conclusion: Direct effect of repeated exposures to hyperbaric air on neural cells likely causes CNS lesions in scuba divers.