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Characterization of clock gene expression and their putative bioactive profiles on development in miniature pig
Author(s) -
Lee Youngjeon Lee,
Won Jinyoung,
Lee Sangkil,
Park Sookyeong,
Hong Yonggeun
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1222.5
Subject(s) - per1 , biology , clock , gene expression , gene , developmental biology , circadian clock , genetics , andrology , medicine
The aim of this study was to address the differential expression of circadian clock genes and their bioactivity profiles on growth in developmental stages of miniature pig (mini‐pig). In growing stages of piglets of either sex on day 0 (isolated from maternal by C‐section), day 1, 5 as well as parturient subjects were sacrificed. Most of all, differences exist in specific clock‐related gene expression between 0‐, 1‐ and 5‐day‐old neonatal and parturient mini‐pig of either sex, especially in brain. We confirmed the differential expression of clock‐related genes in heart and kidney excluding brain at 0‐, 1‐, 5‐day‐old of male mini‐pig. It is giving us the striking message that not only differential expression of clock genes in varies tissues but also dependency of these gene expression on the distinction of sex in neonatal subjects. Additionally, expression of clock, bmal1 and per1 were predominant in kidney compared to brain and heart but not shown any changing their expression on developmental stages. Taken together, therefore, these findings revealed a tissue‐specific differential expression of clock genes at developmental stages that modulates of development of mini‐pigs, suggesting differential physiological function at different developmental stage, especially neonatal periods in perspective donor for xeno‐transplantation. [Supported by BioGreen21 Program (Code#20070401034006), Republic of Korea].