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Mechanosensation and the primary cilium
Author(s) -
Resnick Andrew
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1220.6
Subject(s) - cilium , mechanosensation , chimera (genetics) , microbiology and biotechnology , duct (anatomy) , in vivo , chemistry , biology , anatomy , medicine , ion channel , biochemistry , receptor , gene
The primary cilium has come under increased scrutiny as a site for mechano‐ and chemosensation by cells. We have undertaken a program of study using mouse renal cell lines from the cortical collecting duct and to quantify how mechanical forces arising from fluid shear transduce to a cellular response. Fluid flow through a model nephron has been analysed to determine the in vivo forces. Live cell microscopy is performed to study how the cells respond to both acute and chronic applied mechanical stress. Measured transepithelial ion fluxes are correlated with applied stresses and the cells have been transfected with a GFP chimera shown to localize to the primary cilium.