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Structural damage to erythrocyte‐membrane proteins induced by Lonomia obliqua caterpillar bristle extract
Author(s) -
Seibert Carla Simone,
Tambourgi Denise V.,
Santoro Marcelo L.,
SampaioVerssoni Sandra C,
Takahashi Hilton K.,
Peres Carmem M.,
SanoMartins Ida S.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1219.2
Subject(s) - erythrocyte fragility , hemolysis , chemistry , echinocyte , bristle , lipid bilayer , biochemistry , chromatography , membrane , biology , immunology , materials science , brush , composite material
Lonomia obliqua caterpillar bristle extract shows in vitro hemolytic activity in human and rat erythrocytes (Er), and potent intravascular hemolytic activity in rats. Hemostatic disturbances are characteristic in human envenomation, and intravascular hemolysis have been observed in some cases. Objective To investigate the mechanism whereby L. obliqua caterpillar bristle extract cause lysis of human Er. Methods Human Er were treated with sublytic concentrations (250, 500 and 1000 μg/ml) of crude extract or saline (control). Afterwards, Er osmotic fragility was determined, and sialoglycoproteins of the Er‐membrane surface (GPA, GPC and band 3) were quantified using flow cytometry. Lipid bilayer composition was determined (fatty acids, triacylglycerols, cholesterol). Results Results are expressed as mean± standard deviation (n= 5). Er treated with 1000 μg/ml crude extract increased osmotic fragility (extract H 50% = 90.5 ± 2.70, and control H 50% = 75.0 ± 1.32 mM NaCl). Sialoglycoproteins were removed from the Er surface (26 % GPC and 23 % band 3). The ions Ca 2+ and Mg 2+ increased this activity, showing their greatest effect on band 3 (> 50 % removal). Nevertheless, the extract provoked no damage in the Er lipid bilayer organization. Conclusion Crude extract removes Er membrane proteins responsible for its flexibility and malleability, increasing osmotic fragility, and likely inducing hemolysis by rupturing Er when they are in blood stream. FAPESP