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Effect of Sodium Ferulate on the Cell Migration Induced by TNF‐α
Author(s) -
Zhang Yemin,
Ouyang Jingping,
Zhang Deling,
Wang Fengjie,
Liu Yongming
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1218.8
Subject(s) - cell migration , pseudopodia , tumor necrosis factor alpha , microvillus , microbiology and biotechnology , cell , chemotaxis , cytoplasm , chemistry , biology , medicine , immunology , biochemistry , receptor , membrane
People's Republic of Migration of vascular cells is a crucial response to vascular injury resulting in atherosclerosis. Tumor necrosis factor‐α(TNF‐α) functions as a potent chemoattractant for cells and can change cell cytoskeleton obviously.Sodium Ferulate(SF), a principle from traditional Chinese herb medicine of Angelica, is approved by State Drugs Administration of China as a new drug for the treatment of cardiovascular diseases.This study was to employ ECV304 cells migration model induced by Tumor necrosis factor‐α(TNF‐α) and to make studies of the effect of SF on the migration of ECV304 cells .Through cell scratch wound migration assay and quantification of cell migration, we observed that TNF‐α significantly promoted cell migration of ECV304 and that SF could inhibit the migration induced by TNF‐α treated for 24h.Meanwhile the morphological changes were observed under Giemsa staining and HITACHIS‐570 scanning electron microscope.The morphologies of the migration cells treated only by TNF‐α were markedly changed:cytoplasm radial protruding,numerous pseudopods presenting filament‐like and thin strip‐like contour of some cells presenting.crab.‐ like or.hedgehog.like shape.And the morphologies of the cells treated SF and TNF‐α were similar to the control cells, exhibited regular arrangements, relatively large volume, mostly round or polygon shapes, abundant surface microvillus on the surface.These findings suggest that SF may protect cell pseudopodium and surface microvillus from TNF‐α, and inhibite the migration of ECV304 stimulated by TNF‐α, accordingly it can prevent and treat atherosclerosis.(The research is supported by national natural science foundation of China granted NO.30470680)