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The expression of PKA and VASP within migrating VSMCs in the rat model of type 2 diabetic macroangiopathy
Author(s) -
Zhang DeLing,
Ouyang JingPing,
Zhang YeMin,
Wang Nian,
Su Hui,
Liu YongMing
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1218.3
Subject(s) - medicine , endocrinology , vascular smooth muscle , cytoplasm , phosphoprotein , in vivo , diabetes mellitus , cell , chemistry , biology , phosphorylation , microbiology and biotechnology , smooth muscle , biochemistry
Vasodilator‐stimulated phosphoprotein (VASP), an important substrate of PKA, plays a critical role in the regulation of actin‐based cell motility in vitro . But the role of VASP in cell migration in vivo is not entirely clear. In present study, the relationship between VSMC migration and VASP expression in vivo were investigated. Eight‐week‐old male SD rats were fed with normal diet (control group, n=10) and fed with high fat diet and intraperitoneally injected with STZ (25mg/kg) to induce a model of type 2 diabetes (DM group, n=12). After feeding for 8 weeks, DM group showed significantly increased levels of the plasma glucose, HbAlc and blood cholesterin. Insulin sensitivity was reduced, identified with a glucose test using HOMA‐IR index. Morphological changes of aorta macroangiopathy in DM group were observed by HE staining and transmission electron microscope. The thickness of media was increased and the VSMCs in the media proliferated, the elastic lamella within the intima was destroyed and VSMCs in the media migrated to the intima. Immunohistochemistry showed that expression of PKA and phosphorylated VASP were distributed in the cytoplasm of VSMCs. Compared with diffused distribution within media in control group, the number of VSMCs expressed PKA was increased and mainly distributed in the region near the intima in DM group. Similarly, the distribution of VSMCs expressed VASP was consistent with that of PKA. Our study indicates that the rat model of type 2 diabetic macroangiopathy was successfully established, in which the expression of PKA and phosphorylated VASP were correlated with the migration of VSMCs. (The research is supported by national natural science foundation of China granted NO.30470680)