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Fluorescein transport in isolated renal proximal tubules in experimental diabetic mice
Author(s) -
Lungkaphin Anusorn,
Chatsudthipong Varanuj
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1217.1
Subject(s) - diabetes mellitus , organic anion transporter 1 , renal function , kidney , streptozotocin , endocrinology , chemistry , medicine , lumen (anatomy) , fluorescein , transporter , organic anion transporting polypeptide , biochemistry , fluorescence , physics , quantum mechanics , gene
The renal clearance of organic anions compounds is mediated by organic anion transporters (OATs). Alterations of anion transport function could occur in pathological conditions such as diabetes. However, the role of the renal OATs under this condition has not been assessed. This study examined the transport function of renal OATs in diabetic mice induced by streptozotocin (200 mg/kg BW). The study was performed following 14, 21, 28 and 42 days of diabetic condition to investigate the duration of diabetes on the transport activity of the renal OATs. Fluorescein (FL) was used as a maker of anion transport by OAT1 and 3. Its accumulation in epithelial cells of isolated renal proximal tubules (RPT) was measured using confocal microscopy. We have used quantitative image analysis to follow transport of the organic anion FL from bath (blood side) to lumen in intact mice RPT. It was found that FL accumulation was not significance different from control group (n = 9) and the percentage of FL intensity were 96% and 105% of control, respectively. However, the significance reduction of cellular accumulation of FL was observed in longer period of diabetes, being 62% and 55% of control in 28 (n=5) and 42 (n=3) days of diabetes, respectively. Thus we hypothesize that diabetic condition induced tubular injury and reduced the expression of OAT1 and 3 that affected the transport function of these transporters. However, the mechanisms causing alterations in function and expression of renal OATs need to be further investigated. This work is supported by the Thailand Research Fund grants MRG4980200 and the Faculty of Medicine Endowment Fund, Chiang Mai University.