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VITAMIN C ADMINISTRATION REVERSES BLOOD PRESSURE INCREASE INDUCED BY SUCROSE FEEDING
Author(s) -
Oliveira Thais Galvão Cesar,
Aquino Freitas Raphael Ribeiro,
OliveiraSales Elizabeth Barbosa,
Boim Mirian Aparecida,
Bergamaschi Cássia Toledo,
Dolnikoff Miriam Sterman,
Campos Ruy Ribeiro
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1210.9
Subject(s) - endocrinology , medicine , chemistry , vitamin c , oxidative stress , ascorbic acid , oxidase test , sucrose , messenger rna , enzyme , biology , biochemistry , food science , gene
Sucrose feeding (SF) induces oxidative stress, metabolic abnormalities and high blood pressure (HBP). The aim of this study was to investigate if dietary supplementation with vitamin C ameliorates HBP, hyperinsulinaemia and the mRNA renal expression levels of AT1, NAD(P)H oxidase subunits (p47phox and gp91phox), CuZnSOD induced by SF. Wistar rats (150–180g) were treated, for 30 days, with 20% sucrose solution (SC, n=15) instead of drinking water (CC, n=24). Ascorbic acid (AA, 150 mg/kg) was given by gavage to sucrose‐fed (SAA; n=17) or control rats (CAA; n=25) for the last 7 days of SF. Mean arterial pressure (MAP) was recorded on a Power Lab System. The mRNA expression levels were analyzed in the cortical renal tissue by Real Time RT‐ PCR. An increase (P<0.05) in MAP was observed in SC compared to CC (136±3; 111±4 mmHg). AA supplementation decreases (P<0.05) MAP either in CAA or SAA (102±3; 105±1 mmHg) rats. Basal glucose levels were unchanged but plasma insulin increased (P<0.05) after SF; no alterations were observed after AA. SF increased (P<0.05) p47phox and gp91phox expression but did not change CuZnSOD or AT1 mRNA expression. However, AA reversed the increase of mRNA expression of NAD(P)H oxidase subunits and it reduced (P<0.05) AT1 expression. CuZnSOD expression was unchanged after AA administration. These findings suggest AA ameliorates HBP probably by decreasing oxidative stress induced by SF. Supported by FAPESP (06/58219‐4).