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EFFECTS OF VITAMIN C CHRONIC TREATMENT ON BLOOD PRESSURE, SYMPATHETIC ACTIVITY AND GENE EXPRESSION IN THE RVLM IN RENOVASCULAR HYPERTENSION
Author(s) -
Barbosa OliveiraSales Elizabeth,
Emy Nishi Erika,
Aparecida Boim Mirian,
Sterman Dolnikoff Miriam,
Toledo Bergamaschi Cássia,
Ribeiro Campos Ruy
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1210.4
Subject(s) - rostral ventrolateral medulla , medicine , endocrinology , vitamin c , blood pressure , renovascular hypertension , nad+ kinase , oxidase test , nadph oxidase , messenger rna , mean arterial pressure , chemistry , oxidative stress , enzyme , heart rate , gene , biochemistry
Recently we showed that acute Vitamin C (Vit C) administration (intravenous or directly into the RVLM) caused a significant decrease in mean arterial pressure (MAP) and renal sympathetic nerve activity (RSNA) in 2K‐1C, but not in control rats. Moreover, we found that mRNA expression of NAD(P)H oxidase subunits (p47phox and gp91phox) was greater in 2K‐1C compared to the control group in the RVLM and CuZnSOD expression was similar in both groups. However, the chronic effects of Vitamin C (Vit C) in these parameters are unknown. Therefore, here we treated rats with Vit C (150 mg/kg/day) by gavage for 7 days and MAP, RSNA and mRNA expression of NAD(P)H oxidase subunits (p47phox and gp91phox) and CuZnSOD in the RVLM in the 2K‐1C rats was analyzed. The mRNA expression was analyzed by real‐time PCR. The Vit C resulted in a higher fall in MAP in 2K‐1C than in control group (2K‐1C, 22 ± 7%/C, 11 ± 3%) followed of a significant increase in RSNA (41± 2%) only in control group. The NAD(P)H oxidase subunits expression reduced significantly in both groups treated with Vit C in RVLM. In contrast, CuZnSOD expression was unchanged in all groups. The results support the idea that an increase in oxidative stress within the RVLM probably plays a major role in maintaining hypertension and sympathetic drive in 2K‐1C. Supported by FAPESP.