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Developing an MRI based method for analyzing differences in blood vessel diameter and brain tissue perfusion in hypertension
Author(s) -
Gillies Robert Milne,
Germain Sean,
Raizada Mohan,
Paton Julian,
Walter Glenn
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1210.21
Subject(s) - perfusion , multislice , flip angle , gadolinium , magnetic resonance imaging , medicine , perfusion scanning , magnetic resonance angiography , nuclear medicine , biomedical engineering , chemistry , radiology , organic chemistry
We aimed to develop an MRI based protocol to investigate anatomical differences in brainstem blood vessels between spontaneously hypertensive rats (SHR; n=4) and their normotensive progenitor strain Wistar Kyoto (WKY; n=4) rats. These were compared using high‐resolution multislice, 2D time of flight (TOF) Magnetic Resonance Angiography using different gadolinium (Gd) based contrast agents (Gd‐Albumin, Gadodiamide) to image cerebral blood vessels at 4.7T (TR/TE=18ms/4.3ms, matrix=512x320,nslices=80; nex=4, flip=35°, resolution=68x67x500μm 3 ). Tissue perfusion was measured using dynamic contrast enhancement (TR/TE=12ms/2.8ms, matrix=256x128, nslices=1; nex=1, ΔT=1.5s, images=300, flip=15°, resolution=0.137x0.230x1mm 3 ) or by determining changes in tissue transverse relaxation time pre‐ and post‐contrast enhancement. Images were processed and 3D rendered using Osirix (Mac OS10.4 compatible version). We propose that our protocol will allow measurement and comparison of both blood vessel diameters and tissue perfusion in central cardioregulatory regions in the respective rat strains. Supported by NIH grants HL33610 and 76312.