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Cold Stress Triggered Alterations in Gene Expression in Rat Adrenal Medulla
Author(s) -
Sabban Esther L,
Liu Xiaoping,
Kvetnansky Richard,
Serova Lidia,
Sollas Anne,
Zilinksi Roni
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1205.4
Subject(s) - adrenal medulla , gene expression , biology , microbiology and biotechnology , receptor , signal transduction , endocrinology , gene , medicine , genetics , catecholamine
Prolonged cold stress elicits sensitization of various neuronal systems, including the adrenal medulla (AM) to a novel or heterotypic stressor. To understand the molecular mechanism, a rat cDNA microarray (Affymetrix RAE 230 2.0) was used to analyze the gene expression profile of AM of rats exposed to cold stress (4°C) for 1, 7 or 28 days compared to unstressed controls. The number of transcripts significantly changed >1.5 fold were similar during the cold stress with 46, 69 and 58 up‐regulated and 28, 32, and 25 down‐regulated on days 1, 7 and 28 respectively. Less than half of the up‐regulated, and 20% of the down‐regulated transcripts were changed throughout the 28 days of cold. The greatest increase was seen in those transcripts responsible for proteins involved in gene transcription, secretion/neuropetides, signaling pathways and metabolism and the greatest decrease encompassed these categories as well as those related to channels and chemokines. We confirmed the induction of CREM, bradykinin receptor 2, phospholipase C epsilon, and two factors involved in synaptic vesicle function, VMAT2 and EH domain containing protein. Based on the results, we speculated that up regulation of synaptic vesicle function and/or changes in specific signaling pathways may mediate the sensitization to stress in the AM with cold exposure.