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Daily genistein injections stimulate intestinal chloride secretion in male and female mice via differing mechanisms.
Author(s) -
AlNakkash Layla,
Jensen Jesse,
Hale Nathan,
Batia Lyn
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1202.11
Subject(s) - medicine , endocrinology , basal (medicine) , histaminergic , antagonist , receptor , insulin
Intestinal chloride (Cl) secretion in male and female mice (C57BL/6J) was determined following injection with either 600 mg/kg genistein/day (600G) or genistein‐free (0G, DMSO vehicle control) for either 1 or 2 weeks. After injections, jejuna were isolated and intestinal Cl secretion measured with Ussing chamber short circuit current (Isc, μA/cm 2 ). After 1 week basal Isc was significantly increased in females injected with 600G (198.8±18.9, n=6, P<0.05) compared to 0G (128.3±12.8, n=4). Basal Isc was significantly increased in males injected with 600G (183.6±7.6, n=7) compared to 0G (100.8±18.2, n=7) only after 2 weeks. Cl‐free ringer reduced the basal Isc by 55%, suggesting a major Cl component. Forskolin‐stimulated Isc (10μM bilateral) was significantly increased (P<0.05) in both female and male mice injected with 600G compared to those injected with 0G (n=4–9). Similar data were obtained after 2 weeks of injections. The estrogen‐receptor antagonist ICI‐182780 (25 mg/kg body weight) concomitantly injected with 600G for 2 weeks, significantly decreased basal Isc in males (by 52%, n=3, P<0.01) but not females (by 5%, n=4). Villi length and crypt depth were similar in males and females injected with 600G or 0G. These data suggest that male and female mice respond similarly to 600G (longer duration for males), but the effects are mediated via different mechanisms. Support: CFF and NIH Area grants to L. A.