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Involvement of Epithelial Sodium Channel in Pre‐implantation of Mice
Author(s) -
Ruan Ye Chun,
Chen Wen Ying,
Zhou Wen Liang,
Chan Hsiao Chang
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1201.17
Subject(s) - epithelial sodium channel , amiloride , embryo , chemistry , endocrinology , medicine , immunohistochemistry , andrology , in vivo , sodium , lumen (anatomy) , acid sensing ion channel , epithelium , sodium channel , ion channel , biology , microbiology and biotechnology , pathology , receptor , organic chemistry
Our previous study using semi‐quantitative RT‐PCR has demonstrated that the ENaC expression in mouse uteri was enhanced during pre‐implantation with immunohistochemical data showing their location in endometrial epithelium. We hypothesized that ENaC may play a role in fluid absorption to enhance embryo attachment during pre‐implantation. In the present study, inhibitors of potential ion channels/or transporters were injected to the uterine lumen of mice on day 3 of pregnancy and the number of visible implanted embryo of each mouse was counted on day 8. The results showed that amiloride(10μM), the ENaC inhibitor, was observed to significantly ( p <0.05, n=9) reduce the implanted embryo number to 6.89±1.55 compared to that of the controls (10.64±0.82), while EIPA(10μM), the inhibitor of Na‐H exchanger (NHE), showed no significant effect, indicating the involvement of ENaC rather than NHE. In addition, La 3+ (1mM), a non‐selective cation channel inhibitor, showed no significant effect. These in vivo data together with those observed in our previous studies have demonstrated the involvement of ENaC in the pre‐implantation, indicating a significant role of ENaC in the initiation of implantation.