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Microprojections regulate proliferation and activity of cardiomyocytes derived from mouse embryonic stem cells
Author(s) -
Biehl Jesse K.,
Yamanaka Satoshi,
Boheler Kenneth R.,
Russell Brenda
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1197.6
Subject(s) - embryoid body , embryonic stem cell , chemistry , silicone , biophysics , microbiology and biotechnology , anatomy , biology , biochemistry , induced pluripotent stem cell , gene , organic chemistry
The goal was to test if the physical properties of the microenvironment control proliferation and activity of the mouse embryonic stem cell (mESC) progeny. Microtopographic features were fabricated by photolithography to create 15μm high projections spaced 80 or 500μm apart tetragonally in poly‐dimethyl‐silicone (PDMS) membranes. mESC differentiation began in hanging drops, followed by suspension culture, before the resulting embryoid bodies were disassociated and plated on either flat or the microtextured surfaces. The number of heterogeneous SC derivatives observed with phase microscopy was 60 ± 20% (n=3) on the 80μm microprojections compared to flat PDMS. Similar results were seen for pure cardiomyocytes derived using a puromycin resistant cassette incorporated into the NCX1 promoter. Only 43±12% (n=3) and 75±16% (n=5) of the cardiomyocytes were found on the 80μm and 500μm spaced microprojections, respectively, compared to the flat PDMS. The beating rate per minute of the cardiomyocytes was recorded by video microscopy and was 1.8±0.4 fold higher on the microprojections compared to the flat with surprising changes in coefficients of variance of 0.50 and 0.21 respectively (n=5). Results suggest that microtopography affects both expansion and beating characteristics of mESC progeny. T32HL007692 and HL 62426.