Premium
Analysis of Gene Expression in Human Umbilical Cord Matrix Cells Before and After Differentiation into Ectodermal, Mesodermal and Endodermal Lineages
Author(s) -
Lemming Matthew T.,
Fry Madeline,
Rastovic Milica,
Mitchell Kathy E.
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1197.13
Subject(s) - homeobox protein nanog , biology , stem cell , microbiology and biotechnology , cd90 , cellular differentiation , embryonic stem cell , cd34 , immunology , induced pluripotent stem cell , genetics , gene
ES‐like stem cells from the human umbilical cord matrix (HUCM) express the ES transcription factors Oct‐4 and nanog. HUCM cells also express adult stem cell markers so appear to be intermediate between ES and tissue‐specific adult stem cells. HUCM cells can be differentiated into endodermal, mesodermal and ectodermal lineages making them candidates for cell‐based therapies. We explored changes in gene expression after differentiation of HUCM cells into different cell lineages. In particular, we determined changes in expression of genes associated with undifferentiated embryonic stems (Oct4 and nanog); adult stem cells (CD146, CD90 and smooth muscle actin) and immune complexes (HLA‐ABC and MHC II). We found that Oct4 and nanog expression was decreased to non‐detectable levels after differentiation of HUCM cells and that smooth muscle actin was initially greatly diminished, becoming disorganized and eventually below the level of detection. HLA‐ABC and MHC‐II are not detectable in undifferentiated HUCM cells or in some cases HLA‐ABC expression was very weak. Expression of MHC‐II remained below the level of detection and HLA‐ABC remained very low after in vitro differentiation. This work will establish whether HUCM cells are potential candidates for allogenic cell‐based therapies. Funded by NIH Grant Number P20 RR016475, NIH (R01NS036124) and the State of Kansas.