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LOW SALT DIET DURING PREGNANCY AND LACTATION DEVELOPS OBESITY IN FEMALE ADULT OFFSPRING.
Author(s) -
Lopes Karen Lucasechi,
Furukawa Luzia Naoko Shinohara,
Heimann Joel Claudio
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1196.5
Subject(s) - offspring , lactation , endocrinology , medicine , weaning , leptin , adipose tissue , pregnancy , brown adipose tissue , obesity , biology , genetics
Objective: To evaluate the effect of low salt diet during the perinatal period on adult offspring. Methods: Female Wistar rats were fed normal (NSD 1.3% NaCl) or low (LSD: 0.15% NaCl) salt diet from 8 to 18 weeks of age. At 12 weeks, they were matched. After birth, only 8 pups (4 males ‐ M and 4 females ‐ F) were kept and after weaning all offspring groups were fed standard chow. Body weight (BW) was measured at birth and every week thereafter. Adipose tissue mass and adiposity index (AI), adipocyte volume (AV), plasma leptin level (Lep) were measured in adult offspring (Off). Results (mean&SEM, (p<0.05) (n= 5): BW (g) was lower at the birth in LSD (M‐5.6±0.1 and F‐5.8±0.1) than in NSD Off (M‐6.5±0.1, F‐6.2±0.1). The Lep (ng/mL) was higher in LSD (5.3±0.1) than in NSD M Off (1.9±0.4), otherwise in LSD Lep was lower (1.7±0.6) than in NSD F Off (3.7±0.6) group. The AI was higher in LSD (8.3±0.6) compared to NSD F Off (6.3±0.6), but not in M Off. The retroperitoneal (RET) and brown adipose (BAT) tissue mass (g) were higher in LSD F Off compared to NSD F Off. AV (μm 3 ) was higher in RET and lower in BAT in LSD compared to NSD F Off, but not in M Off. Conclusions: sex‐specific effects of salt restriction during the perinatal period on adult fat content and distribution were observed. These results are important, once they unraveled a previously unknown mechanism of intrauterine programming of adult obesity. Supported by FAPESP.