Premium
Molecular cloning, distribution and developmental regulation of porcine GLUT2 mRNA in small intestine
Author(s) -
Huang Zhiyi,
Feng Dingyuan,
Zou Shigeng,
Zhi Aimin,
Zuo Jianjun
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1193.2
Subject(s) - glut2 , small intestine , biology , messenger rna , ileum , jejunum , weaning , cloning (programming) , large intestine , complementary dna , andrology , medicine , microbiology and biotechnology , endocrinology , gene expression , gene , biochemistry , computer science , programming language
Molecular cloning, tissue distribution and developmental regulation of porcine GLUT2 (glucose transporter 2) mRNA were conducted in small intestine. GLUT2 cDNA is 2051 bp long, the predicted amino acid sequence revealed that it is highly conserved among vertebrate species. Animal tissue samples were collected from 35 pigs at different developmental stages: sucking (day 1, 7 and 26), postweaning (day 30) and growing (day 60, 90 and 150). The results showed that GLUT2 mRNA was detected at highest level in porcine liver, at moderate levels in the small intestine, kidney, and at low levels in the brain, lung, muscle and heart. In the small intestine, the highest level is in the jejunum. After weaning, the three small intestinal sements took on a similar pattern, GLUT2 mRNA increased through post‐weaning (d30, 2 days post‐weaning) and peaked in growing period (d90), and then decreased in finishing period (d150). In conclusion, the mRNA expression of GLUT2 was not only differentially regulated by age but also differentially distributed along the small intestine of piglets at different stages, which may be related to intestinal luminal substrate concentrations. This study was supported by the National Basic Research Program of China (Project No: 2004CB117501) and National Natural Science Foundation of China (Project No: 30671529).