Premium
Estrogen Augments Shear Stress‐induced Signaling and Gene Expression in Osteoblasts
Author(s) -
Yeh ChiuanRen,
Cheng ChengKung,
Chiu JengJiann,
Chien Shu
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1188.12
Subject(s) - estrogen , mapk/erk pathway , signal transduction , osteoblast , p38 mitogen activated protein kinases , endocrinology , chemistry , medicine , gene expression , microbiology and biotechnology , biology , gene , biochemistry , in vitro
Estrogen and fluid shear stress play important roles in the pathogenesis of osteoporosis (OP). We investigated the role of interplays between estrogen and shear stress in signal transduction and gene expression in osteoblasts. Both estrogen and shear stress induce transient phosphorylations of extracellular signal‐regulated kinase (ERK) and p38 and expressions of cyclooxygenase‐2 (Cox‐2) and c‐fos in MG63 osteoblast‐like cells. Treatment of MG63 cells with ERK and p38 inhibitors inhibited the estrogen‐induced Cox‐2 and c‐fos expressions. Application of MG63 cells treated with estrogen for 6 h to shear stress (12 dynes/cm2) for 30 min significantly induced increases in ERK and p38 phosphorylations and Cox‐2 and c‐fos expressions, as compared with the cells without estrogen, suggesting estrogen augments the shear stress ‐induced signaling and gene expression in MG63 cells. MG63 cells treated with estrogen for 6 h induced their expression of beta1 integrin, which acts as a mechanoreceptor contributing to estrogen ‐enhancement of signaling and gene expression in MG63 cells in response to shear. Our results suggest that interplays of estrogen and mechanical force exert additive effects on osteoblast responses.