Premium
Genetically diverse invasive E. coli from IBD patients induce high concentrations of TNF‐α
Author(s) -
Klapproth JanMichael Axel,
Ribot Efrain,
GernerSmidt Peter,
Garrett Nancy,
Sasaki Maiko
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1183.9
Subject(s) - escherichia coli , biology , genotype , population , tumor necrosis factor alpha , microbiology and biotechnology , macrophage , phenotype , ulcerative colitis , immunology , gene , in vitro , disease , medicine , pathology , genetics , environmental health
Increased number of invasive E. coli has been identified in patients with Crohn's disease (CD). Our goal was to characterize genotype and phenotype of invasive E. coli in American population of patients with CD, ulcerative colitis (UC) and normal controls. Significant genetic similarities, as determined by pulse field gel electrophoresis and cluster analysis, were identified for 114 E. coli strains from the same patient, regardless of whether they were isolated from macroscopically inflamed or normal appearing tissues (cluster II 95.5%, cluster III 81.3%). Invasive isolates from different patients were not genetically related but all IBD E. coli strains induced high mean concentrations of TNF‐α (CD 2,607.6 pg ±450/1 X 10 5 cells, UC 2,374.7 pg ±54/1 X 10 5 cells) and IL‐8 (CD 850.1 pg ±107/1 X 10 5 cells, UC 1,089 pg ±54/1 X 10 5 cells) in macrophage and epithelial cell cultures respectively. In comparison to isolates from normal tissues, invasive E. coli isolated from inflamed CD samples induced more TNF‐α (inflamed 3,081.4 ±235/1 X 10 5 cells, normal CD 2,476.5 pg ±448/1 X 10 5 cells), but significantly less IL‐8 (inflamed 672.2 ±59/1 X 10 5 cells, normal 992.2 pg ±94/1 X 10 5 cells) in cell cultures. TNF‐á expression was similar in TLR‐4 deficient and wild type macrophage cultures. Even though genetically diverse, phenotypically, invasive E. coli from patients with CD induce high concentrations of TNF‐α.