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Cilia‐mediated heat shock protein 27 (hsp27) suppression modulates actin polymerization and organization in wild type mouse and oak ridge polycystic kidney (orpk) cells
Author(s) -
Jones Thomas Joseph,
Mell Blair M,
Dokas Linda A,
Yoder Bradley K,
Nauli Surya M
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1177.2
Subject(s) - cilium , hsp27 , cytoskeleton , microbiology and biotechnology , actin cytoskeleton , actin , biology , heat shock protein , hsp70 , cell , biochemistry , gene
Primary cilia play a pivotal role in the development of polycystic kidney disease (PKD), a state with an enhanced propensity for hypertension. Cilia, characterized as mechano/chemo‐sensing organelles, are uniquely situated to communicate extracellular signals to intracellular responses. Because cytoskeletal remodeling plays a significant role in cellular adaptation, we postulated that stress‐induced hsp27, which associates with actin to stabilize the cytoskeleton, could mediate this response. To that end, we evaluated shear and oxidative stress on hsp27 levels and actin polymerization in cilia deficient endothelial cells isolated from the orpk model of PKD. By immunoblotting orpk had a basal suppression of hsp27 compared to wild‐type (wt) cells. Basal suppression could be overcome by oxidative stress but not shear stress. Basal actin levels were consistently elevated in orpk when compared to wt cells and stress had a minimal effect on these levels. Actin organization was inversely correlated with hsp27 levels. Our observations provide evidence that disruption of cilia formation suppresses basal hsp27 levels by an undetermined mechanism and increases the unorganized state of actin. Results suggest primary cilia have a role in regulating actin organization and in providing cytoskeletal adaptation to stress potentially through hsp27. (AHA‐0630257N, NIH‐ HL084451 )