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Exercise Combined with Hypoxia Modulates Cytotoxicity of Natural Killer Cells to Nasopharyngeal Carcinoma Cells
Author(s) -
Wu ChiaKuan,
Wang Jongshyan
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1173.4
Subject(s) - perforin , cytotoxicity , hypoxia (environmental) , nasopharyngeal carcinoma , granzyme , apoptosis , natural killer cell , granzyme b , medicine , immunology , biology , chemistry , oxygen , in vitro , biochemistry , organic chemistry , radiation therapy
This study investigated how exercise combined with hypoxia affects the cytotoxicity of natural killer cells (NK) to nasopharyngeal carcinoma cells (NPC) and its underlying mechanism. Eight sedentary men engaged six conditions in a normobaric hypoxia chamber for 1 hour: two normoxic exercise [i.e., strenuous exercise (SE, up to VO2max) and moderate exercise (ME, 50%VO2max) in 21% O2], two hypoxic exercise (i.e., ME in 12% and 15% O2), and two hypoxic conditions (i.e., 12% and 15% O2). Our results showed that SE induced higher NK cell count and perforin/granzyme levels and NK‐induced apoptosis of NPC than ME. However, combined with 15% O2 intervention, ME promoted extent of NK‐induced apoptosis of NPC, accompanied by augmenting the increases of NK cell count and perforin level by this exercise. Although ME combined with 12% O2 intervention increased NK count and perforin level, no significant change occurred in cytotoxicity of NK to NPC following this intervention. Therefore, we conclude that ME combined with 15% O2, but not 12% O2, intervention enhances NK count and perforin level and subsequently promotes the cytotoxicity of NK to NPC.