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Network of Paraventricular Nucleus Gene Expression in Angiotensin‐salt Hypertension
Author(s) -
Yuan Lihui,
Li Xia,
Sved Judith,
Sved Alan,
Liu Li,
Raizada Mohan
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1168.9
Subject(s) - medicine , endocrinology , angiotensin ii , gene expression , nucleus , receptor , gene , tumor necrosis factor alpha , pathogenesis , renin–angiotensin system , biology , blood pressure , microbiology and biotechnology , genetics
Neurons in the hypothalamic paraventricular nucleus (PVN) are critical in control of cardiovascular function and their altered activity may contribute to the pathogenesis of hypertension. In this study we used gene expression profiling to identify PVN genes that may contribute to hypertension produced by the interaction of angiotensin II (AngII) and high dietary salt. Hypertension was established in male Sprague Dawley rats by infusion of AngII (150 ng/kg/hr, sc) in combination with a 2% NaCl diet; control rats were fed a 0.1 % NaCl diet and did not receive AngII. After 13 days of treatment, brains were collected and the PVN isolated and analyzed using Agilent gene array chips. Significantly regulated genes (ANOVA, p<0.05) were evaluated using PathwayStudio ® software to reveal functional network relationships. A gene network revealed transcriptional regulation patterns centered around tumor necrosis factor (TNF). PVN expression of TNF was decreased in hypertensive rats and is positioned in a pivotal role that directly or indirectly regulates expression of other hypertension‐related genes. This network also involves several transcriptional factors (MLLT1, TLX2) and receptors (ITGAM, HTR1 A). Our data suggest that decreased PVN expression of TNF plays a hub role in network of changes in gene expression associated with hypertension. (Supported by HL76083)