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Gene Expression Networks in Rostral Ventrolateral Medulla (RVLM) of Rats with Angiotensin‐salt Hypertension
Author(s) -
Yuan Lihui,
Li Xia,
Sved Judith,
Sved Alan,
Raizada Mohan,
Liu Li
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1168.10
Subject(s) - rostral ventrolateral medulla , medicine , angiotensin ii , endocrinology , blood pressure , renin–angiotensin system , angiotensin receptor , chemistry , heart rate
The rostral ventrolateral medulla (RVLM) is essential in neural control of cardiovascular function and changes in RVLM may play an important role in hypertension. In this study expression profiling was used to identify RVLM genes that may contribute to hypertension produced by the interaction of angiotensin II (AngII) and high dietary salt. Hypertension was established in male Sprague Dawley rats by infusion of AngII (150 ng/kg/hr, sc) in combination with a 2% NaCl diet; control rats were fed a 0.1% NaCl diet and did not receive AngII. After 13 days of treatment, which increased blood pressure by ~30 mm Hg, RVLM were collected and analyzed using Agilent gene array chips. Significantly regulated genes (ANOVA, p<0.05) were evaluated using PathwayStudio ® software to reveal functional network relationships. A large gene network was revealed with regulatory hubs centered around several genes including CASP3 (caspase 3), HSPA1A (heat shock 70kD protein 1B), EGFR (epidermal growth factor receptor), and NR3C1 (nuclear receptor). In addition, many genes in the network, including transcription factors TP73L, RUNX2 and DAXX, oxidoreductase CYP4A11 and CYP21A2, have previously been implicated either directly or indirectly in hypertension. These results provide a framework to begin to understand molecular changes in the RVLM associated with hypertension. (Supported by HL76083)