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Central and peripheral leptin treatment produce similar increase in muscle and bone mass in ob/ob mice
Author(s) -
Bartell Shoshana M,
Gaddam Dhanunjaya R,
Ambati Suresh,
Hartzell Diane L,
Hamrick Mark,
She JinXiong,
DellaFera Mary Anne,
Baile Clifton A
Publication year - 2008
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.22.1_supplement.1166.1
Subject(s) - leptin , medicine , endocrinology , fat mass , chemistry , bone mineral , skeletal muscle , bone density , body weight , obesity , osteoporosis
Leptin administered either centrally or peripherally reduces body weight (BW), food intake (FI), & body fat in leptin deficient ob/ob mice. However, there are contradictory findings when assessing effects of leptin on bone mass in rodents. The objective of this study was to determine the effects of peripheral & central administration of leptin on bone metabolism in the axial & appendicular skeleton in ob/ob mice (15 wk old, init. BW=61.3g). Leptin was delivered subcutaneously (SQ, 0 or 10μg/d) or intracerebroventricularly (ICV, 0 or 1.5μg/d) via osmotic pumps for 12 days (n=10). There were no significant differences between ICV and SQ leptin. Regardless of mode of administration, leptin decreased BW (51.0 vs 62.2g, p=0.003; mean of leptin treatments vs controls), FI (3.85 vs 5.69g, p=0.03), body fat (46.6 vs 56.0g, p=0.001), & relative total fat pad wt (13.1 vs 12.9g/g BW p=0.02) & increased gastrocnemius muscle wt (97.8 vs 110.4mg, p=0.01), relative muscle mass (242.7 vs 173.4mg/g BW, p<0.01), bone mineral density (0.0554 vs 0.0510g/cm 2 , p=0.001), & bone mineral content (0.418 vs 0.376g, p=0.02). These results show that both modes of leptin administration increased bone & muscle mass equally in ob/ob mice. Supported by the Georgia Research Alliance (GRA) Eminent Scholar endowment (CAB) & a GRA Challenge Grant (CAB & JXS).